FAT METABOLISM IN HIGHER PLANTS

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Fat Metabolism in Higher Plants

The discovery and isolation of the multienzyme system responsible for p oxidation of fatty acids to acetyl CoA’ have stimulated investigation of the synthesis of long chain fatty acids by multiple @ condensation of the acetyl CoA. In 1953 Van Baalen and Gurin (1) were the first to demonstrate that acetate was incorporated into fatty acids by aqueous extracts of pigeon liver. In 1953 Stansly and...

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Fat Metabolism in Higher Plants XXXVI: Long Chain Fatty Acid Synthesis in Germinating Peas.

A low lipid, high starch containing tissue, namely cotyledons of germinating pea seedlings was examined for its capacity to synthesize fatty acid. Intact tissue slices readily incorporate acetate-(14)C into fatty acids from C(16) to C(24). Although crude homogenates synthesize primarily 16:0 and 18:0 from malonyl CoA, subsequent fractionation into a 10,000g pellet, a 10(5)g pellet and supernata...

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Fat metabolism in higher plants. VIII. Saturated long chain fatty acid peroxidase.

In a previous communication (1) it was reported that an L-a-hydroxy aliphatic acid was required as a cofactor for the limited oxidation of palmitic acid by a soluble cytoplasmic protein fract,ion obtained by precipitation with ammonium sulfate from extracts of cotyledons of germinating peanuts. This fraction was designated supernatant enzyme. No additional cofactors were required, and activity ...

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Fat Metabolism in Higher Plants. Vii. 8-oxidation of Fatty Acids by Peanut Mitochondria1,

The concept of 8-oxidation of fatty acids has been well documented in animal tissues. In 1943 Munoz and Leloir (12) prepared cell-free systems from rat liver that oxidized butyric acid to CO2 and water. Kennedy and Lehninger (10) later showed that the site of fatty acid oxidation is the mitochondrion. Green and his co-workers (15) and Lynen (11) prepared soluble enzymes from acetone powders of ...

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Fat Metabolism in Higher Plants: LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts.

Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were ei...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1956

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)65063-x